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2-羟基丁酸 1

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功能代谢组学 1

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除草剂 1

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Effects of iron oxide nanoparticles on phenotype and metabolite changes in hemp clones ( L.)

《环境科学与工程前沿（英文）》 2022年第16卷第10期 doi: 10.1007/s11783-022-1569-9

摘要：

● Fe₃O₄ NPs increased the biomass and chlorophyll content of hemp clones.

关键词： Fe₃O₄ nanoparticle Hemp Growth enhancement THC Metabolite

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METABOLIC AND TRANSCRIPTOME ANALYSIS REVEALS METABOLITE VARIATION AND FLAVONOID REGULATORY NETWORKS IN

《农业科学与工程前沿（英文）》 2021年第8卷第2期

摘要：

Metabolites, especially secondary metabolites, are very important in the adaption of tea plants and the quality of tea products. Here, we focus on the seasonal variation in metabolites of fresh tea shoots and their regulatory mechanism at the transcriptional level. The metabolic profiles of fresh tea shoots of 10 tea accessions collected in spring, summer, and autumn were analyzed using ultra-performance liquid chromatography coupled with quadrupole-obitrap mass spectrometry. We focused on the metabolites and key genes in the phenylpropanoid/flavonoid pathway integrated with transcriptome analysis. Multivariate statistical analysis indicates that metabolites were distinctly different with seasonal alternation. Flavonoids, amino acids, organic acids and alkaloids were the predominant metabolites. Levels of most key genes and downstream compounds in the flavonoid pathway were lowest in spring but the catechin quality index was highest in spring. The regulatory pathway was explored by constructing a metabolite correlation network and a weighted gene co-expression network.

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METABOLIC AND TRANSCRIPTOME ANALYSIS REVEALS METABOLITE VARIATION AND FLAVONOID REGULATORY NETWORKS IN

Chen-Kai JIANG, De-Jiang NI, Ming-Zhe YAO, Jian-Qiang MA, Liang CHEN

《农业科学与工程前沿（英文）》页码 215-230 doi: 10.15302/J-FASE-2021382

摘要： Metabolites, especially secondary metabolites, are very important in the adaption of tea plants and the quality of tea products. Here, we focus on the seasonal variation in metabolites of fresh tea shoots and their regulatory mechanism at the transcriptional level. The metabolic profiles of fresh tea shoots of 10 tea accessions collected in spring, summer, and autumn were analyzed using ultra-performance liquid chromatography coupled with quadrupole-obitrap mass spectrometry. We focused on the metabolites and key genes in the phenylpropanoid/flavonoid pathway integrated with transcriptome analysis. Multivariate statistical analysis indicates that metabolites were distinctly different with seasonal alternation. Flavonoids, amino acids, organic acids and alkaloids were the predominant metabolites. Levels of most key genes and downstream compounds in the flavonoid pathway were lowest in spring but the catechin quality index was highest in spring. The regulatory pathway was explored by constructing a metabolite correlation network and a weighted gene co-expression network.

关键词： harvest season metabolites tea shoots transcriptomics untargeted metabolomics

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Water-dispersible nano-pollutions reshape microbial metabolism in type-specific manners: A metabolic and bacteriological investigation in

《环境科学与工程前沿（英文）》 2022年第16卷第9期 doi: 10.1007/s11783-022-1548-1

摘要：

• Water-dispersible nano-pollutions exhibit type-specific toxic effects on E. coli.

关键词： Nano-toxicity Nano-plastics Quantum dots Microbial metabolite Metabolic dysregulation

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通过定量分析植物初级代谢产物和酶代谢库发现除草剂新作用位点 Perspective

Franck E. Dayan, Stephen O. Duke

《工程（英文）》 2020年第6卷第5期页码 509-514 doi: 10.1016/j.eng.2020.03.004

摘要：

自20世纪80年代以来，具有新分子作用位点（SOA）的除草剂一直没有被发现。从那时起，杂草对大多数商用除草剂产生了广谱抗性，这极大地增加了对具有新SOA的除草剂的需求。本文讨论了两种未被尝试过的方法，用于发现除草剂的新SOA。一些初级代谢中间产物（如原卟啉IX和鞘氨醇碱）对植物有毒性，因此，在植物体内，这些化合物的浓度水平非常低。确定所有初级代谢产物的植物毒性和代谢库大小，有利于识别相关SOA。本文讨论的发现除草剂的第一种新方法是研究导致植物毒性化合物积累的SOA，第二种方法是识别体内酶水平非常低的潜在SOA。我们知道，SOA的酶分子数量越多，杀死植物所需的除草剂就越多。现代蛋白质组学方法可以识别酶水平较低的SOA，为除草剂的发现提供依据。这些方法可能有助于发现与天然化合物更紧密相关并且可以在低剂量下使用的除草剂。

关键词：天然产物合理的生物发现作用方式除草剂

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功能代谢组学揭示黄芪多糖通过2-羟基丁酸改善肥胖小鼠的脂质代谢 Article

李冰冰, 洪颖, 顾彧, 叶圣洁, 胡凯莉, 姚建, 丁侃, 赵爱华, 贾伟, 李后开

《工程（英文）》 2022年第9卷第2期页码 111-122 doi: 10.1016/j.eng.2020.05.023

摘要：

多糖广泛存在于草本植物中，具有多种活性，特别是具有免疫调节作用和改善代谢紊乱的作用。然而，其潜在机制还没有被很好地诠释。功能代谢组学越来越多地被用于通过鉴定具有特定功能的代谢产物来研究多糖对宿主的系统性影响。本研究采用功能代谢组学策略，探讨黄芪多糖（Astragalus polysaccharide, APS）代谢获益的潜在机制。在为期8 周的高脂肪饮食（high-fat diet, HFD）喂养的肥胖小鼠中测定了APS的作用。然后，进行基于气相色谱-飞行时间质谱（gas chromatography-time-of-flight mass spectrometry, GC-TOFMS）的非靶向代谢组学分析血清和肝组织，并进行基于液相色谱- 串联质谱（liquid chromatography-tandem mass spectrometry, LC-MS/MS）的靶向代谢组学分析。使用体外和体内代谢紊乱模型测试了代谢产物的潜在功能。我们的结果首次证实了APS对肥胖小鼠的代谢益处。然后，代谢组学分析显示，补充APS 可逆转HFD 诱导的代谢变化，并确定2-羟基丁酸（2-hydroxybutyric acid, 2-HB）是APS活性的潜在功能性代谢产物，该代谢物被HFD显著降低，并被APS逆转。进一步研究表明，2-HB可抑制油酸（oleic acid, OA）诱导的甘油三酯（triglyceride, TG）累积。还发现其可刺激肝细胞中脂质降解蛋白的表达和3T3-L1 细胞中的TG脂解。此外，在高脂肪和高蔗糖（high-fat and high-sucrose, HFHS）喂养的小鼠中发现2-HB可降低血清TG并调节参与脂质降解的蛋白质。总之，我们的研究表明，APS的代谢
益处至少部分归因于2-HB 的产生，2-HB 在体外和体内均可调节脂质代谢。我们的结果同样证实，功能代谢组学在研究植物多糖系统益处的潜在机制方面是切实可行的。

关键词：黄芪多糖功能代谢组学 2-羟基丁酸肥胖